This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
hIRE1a K599A
catalog :
20745
citations: 2
Reference
Lim M, Devi L, Rozenfeld R. Cannabidiol causes activated hepatic stellate cell death through a mechanism of endoplasmic reticulum stress-induced apoptosis. Cell Death Dis. 2011;2:e170 pubmed publisher
Lipson K, Ghosh R, Urano F. The role of IRE1alpha in the degradation of insulin mRNA in pancreatic beta-cells. PLoS ONE. 2008;3:e1648 pubmed publisher
product information
Catalog Number :
20745
Product Name :
hIRE1a K599A
article :
doi10.1371/journal.pone.0001648
id2497
pubmed_id18286202
bacterial resistance :
Ampicillin
cloning :
backbonepLenti CMV/TO Puro DEST (670-1)
backbone_mutation
backbone_originEric Campeau (Addgene plasmid 17293)
backbone_size9594
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Other
Destination vector
growth notes :
IRE1a cDNA was cut out of pENTR1A with EcoRI and XhoI and ligated into pLenti CMV/TO Puro DEST through Gateway cloning.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3Gateway
cloning_site_5Gateway
promoter
sequencing_primer_3MSCV-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesIRE1, IRE1P, IRE1a, hIRE1p
geneERN1
id2081
genbank_ids
mutationK599A, dominant negative kinase mutant
nameIRE1
shRNA_sequence
size2934
species
9606
Homo sapiens
tags
resistance markers :
540
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA