This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
hIRE1a wt
catalog :
20744
citations: 6
Reference
Rohde C, Becker S, Krähling V. Marburg virus regulates the IRE1/XBP1-dependent unfolded protein response to ensure efficient viral replication. Emerg Microbes Infect. 2019;8:1300-1313 pubmed publisher
Wheeler M, Jaronen M, Covacu R, Zandee S, Scalisi G, Rothhammer V, et al. Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation. Cell. 2019;176:581-596.e18 pubmed publisher
Fink S, Jayewickreme T, Molony R, Iwawaki T, Landis C, Lindenbach B, et al. IRE1α promotes viral infection by conferring resistance to apoptosis. Sci Signal. 2017;10: pubmed publisher
Qi Y, Wang W, Chen J, Dai L, Kaczorowski D, Gao X, et al. Sphingosine Kinase 1 Protects Hepatocytes from Lipotoxicity via Down-regulation of IRE1α Protein Expression. J Biol Chem. 2015;290:23282-90 pubmed publisher
Son S, Byun J, Roh S, Kim S, Mook Jung I. Reduced IRE1? mediates apoptotic cell death by disrupting calcium homeostasis via the InsP3 receptor. Cell Death Dis. 2014;5:e1188 pubmed publisher
Lipson K, Ghosh R, Urano F. The role of IRE1alpha in the degradation of insulin mRNA in pancreatic beta-cells. PLoS ONE. 2008;3:e1648 pubmed publisher
product information
Catalog Number :
20744
Product Name :
hIRE1a wt
article :
doi10.1371/journal.pone.0001648
id2497
pubmed_id18286202
bacterial resistance :
Ampicillin
cloning :
backbonepLenti CMV/TO Puro DEST (670-1)
backbone_mutation
backbone_originEric Campeau (Addgene plasmid 17293)
backbone_size9594
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Other
Destination vector
growth notes :
IRE1a cDNA was cut out of pENTR1A with EcoRI and XhoI and ligated into pLenti CMV/TO Puro DEST through Gateway cloning.
growth temp :
DB3.1 or ccDB survival, 37oC
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3Gateway
cloning_site_5Gateway
promoter
sequencing_primer_3MSCV-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesIRE1, IRE1P, IRE1a, hIRE1p
geneERN1
id2081
genbank_ids
mutation
nameIRE1
shRNA_sequence
size2934
species
9606
Homo sapiens
tags
resistance markers :
540
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA