PET30B-PTEN | Addgene 20741 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PET30B-PTEN

catalog :

20741

citations: 4

Reference
Dawson J, SMITH I, Martin W, Khan K, Cheng F, Eng C. Shape shifting: The multiple conformational substates of the PTEN N-terminal PIP2 -binding domain. Protein Sci. 2022;31:e4308 pubmed publisher
Johnston S, Raines R. Conformational stability and catalytic activity of PTEN variants linked to cancers and autism spectrum disorders. Biochemistry. 2015;54:1576-82 pubmed publisher
Johnston S, Raines R. Catalysis by the tumor-suppressor enzymes PTEN and PTEN-L. PLoS ONE. 2015;10:e0116898 pubmed publisher
Redfern R, Redfern D, Furgason M, Munson M, Ross A, Gericke A. PTEN phosphatase selectively binds phosphoinositides and undergoes structural changes. Biochemistry. 2008;47:2162-71 pubmed publisher

product information

Catalog Number :

20741

Product Name :

PET30B-PTEN

article :

doi	10.1021/bi702114w
id	2494
pubmed_id	18220422

bacterial resistance :

Kanamycin

cloning :

backbone

PET30b

backbone_mutation

backbone_origin

Novagen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

The cDNA encoding full-length (1-403) human PTEN was cloned into the NdeI and XhoI sites of the pET30b vector, thereby introducing a six histidine tag at the C-terminus.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NdeI
promoter
sequencing_primer_3	T7 terminator
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1
gene	PTEN
id	5728

genbank_ids

NM_000314

mutation

name

PTEN

shRNA_sequence

size

species

9606

Homo sapiens