This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHJ322
catalog :
20677
citations: 3
Reference
Mangon A, Salaün D, Bouali M, Kuzmic M, Quitard S, Thuault S, et al. iASPP contributes to cell cortex rigidity, mitotic cell rounding, and spindle positioning. J Cell Biol. 2021;220: pubmed publisher
Beaty B, Wang Y, Bravo Cordero J, Sharma V, Miskolci V, Hodgson L, et al. Talin regulates moesin-NHE-1 recruitment to invadopodia and promotes mammary tumor metastasis. J Cell Biol. 2014;205:737-51 pubmed publisher
Hao J, Liu Y, Kruhlak M, DeBell K, Rellahan B, Shaw S. Phospholipase C-mediated hydrolysis of PIP2 releases ERM proteins from lymphocyte membrane. J Cell Biol. 2009;184:451-62 pubmed publisher
product information
Catalog Number :
20677
Product Name :
pHJ322
article :
doi10.1083/jcb.200807047
id2478
pubmed_id19204146
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth temp :
DH5a
origin :
37
pi :
alt_names
MSN
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoter
sequencing_primer_3GATGAGCAGGATGAGAAT
sequencing_primer_5GTGCATAAGTCTGGCTAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL70, IMD50
geneMSN
id4478
genbank_ids
NM_002444
mutationChanged Thr 558 to Asp
nameMoesin
shRNA_sequence
size1731
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagGFP
resistance markers :
465
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA