pWZL Neo Myr Flag RET | Addgene 20614 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pWZL Neo Myr Flag RET

catalog :

20614

citations: 2

Reference
Quintela Fandino M, Bueno M, Lombardia L, Gil M, Gonzalez Martin A, Marquez R, et al. Selective activity over a constitutively active RET-variant of the oral multikinase inhibitor dovitinib: results of the CNIO-BR002 phase I-trial. Mol Oncol. 2014;8:1719-28 pubmed publisher
Boehm J, Zhao J, Yao J, Kim S, Firestein R, Dunn I, et al. Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007;129:1065-79 pubmed

product information

Catalog Number :

20614

Product Name :

pWZL Neo Myr Flag RET

article :

doi	10.1016/j.cell.2007.03.052
id	2406
pubmed_id	17574021

bacterial resistance :

Ampicillin

cloning :

backbone

pWZL-Neo-Myr-Flag-DEST

backbone_mutation

backbone_origin

William Hahn Lab (available at Addgene, #15300)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Addgene's sequencing results identified a R982C mutation when compared to GenBank ID NP_065681.1. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021

growth temp :

Stbl3 cells to prevent recombination.

origin :

pi :

alt_names

ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)

RET9

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	pWZL-Rev (TTC CGG GCC CTC ACA TTG)
sequencing_primer_5	pWZL-Fwd (GAA CCT CCT CGT TCG ACC)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1
gene	RET
id	5979

genbank_ids

NM_020630

mutation

R982C

name

RET

shRNA_sequence

size

species

9606

Homo sapiens