This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
An R57A, R59A CAAX pMT2
catalog :
206119
citations: 1
Reference
Page K, Heblich F, Margas W, Pratt W, Nieto Rostro M, Chaggar K, et al. N terminus is key to the dominant negative suppression of Ca(V)2 calcium channels: implications for episodic ataxia type 2. J Biol Chem. 2010;285:835-44 pubmed publisher
product information
Catalog Number :
206119
Product Name :
An R57A, R59A CAAX pMT2
article :
doi10.1074/jbc.M109.065045
id28238450
pubmed_id19903821
bacterial resistance :
Ampicillin
cloning :
backbonepMT2
backbone_mutation
backbone_originGenetics Institute Inc
backbone_size5163
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif
origin :
37
pi :
alt_names
Cav2.1 alpha1A
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5unknown
promoterAd MLP/TPL/SV40
sequencing_primer_3GGTCGAACCATGATGGCAGC
sequencing_primer_5AGCTTGAGGTGTGGCAGGCTT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBccA1, Cav2.1, rbA-1
geneCacna1a
id25398
genbank_ids
mutation R57A, R59A
namecacna1a
shRNA_sequence
size346
species
10116
Rattus norvegicus
tags
plasmid copy :
Terry P. Snutch
resistance markers :
2017
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA