This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cav2.2 HA EIVK (E1658K) pCAGGS
catalog :
206096
citations: 1
Reference
Meyer J, Dahimene S, Page K, Ferron L, Kadurin I, Ellaway J, et al. Disruption of the Key Ca2+ Binding Site in the Selectivity Filter of Neuronal Voltage-Gated Calcium Channels Inhibits Channel Trafficking. Cell Rep. 2019;29:22-33.e5 pubmed publisher
product information
Catalog Number :
206096
Product Name :
Cav2.2 HA EIVK (E1658K) pCAGGS
article :
doi10.1016/j.celrep.2019.08.079
id28209835
pubmed_id31577951
bacterial resistance :
Ampicillin
cloning :
backbonepCAGGS
backbone_mutation
backbone_originPMID 11397804
backbone_size4890
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
5' end of DNA is very GC rich
growth strain :
expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface
origin :
37
pi :
alt_names
Cav2.2 alpha1B
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5unknown
promoterCMV/B-actin
sequencing_primer_3CTGATAGGCAGCCTGCACCTG
sequencing_primer_5CTCTGCTAACCATGTTCATGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCACNA1B
id100008979
genbank_ids
mutationE1658K
namecacna1b
shRNA_sequence
size7160
species
9986
Oryctolagus cuniculus
tags
plasmid copy :
Yasuo Mori
resistance markers :
2017
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA