GFP Cav2.2 W391A pRK5 | Addgene 206084 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

GFP Cav2.2 W391A pRK5

catalog :

206084

citations: 1

Reference
Waithe D, Ferron L, Page K, Chaggar K, Dolphin A. Beta-subunits promote the expression of Ca(V)2.2 channels by reducing their proteasomal degradation. J Biol Chem. 2011;286:9598-611 pubmed publisher

product information

Catalog Number :

206084

Product Name :

GFP Cav2.2 W391A pRK5

article :

doi	10.1074/jbc.M110.195909
id	28238454
pubmed_id	21233207

bacterial resistance :

Ampicillin

cloning :

backbone

pRK5

backbone_mutation

backbone_origin

backbone_size

4716

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

5' end of Cav2.2 DNA is very GC rich

growth strain :

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

origin :

pi :

alt_names

Cav2.2 alpha1B

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5	unknown
promoter	CMV
sequencing_primer_3	TGAGTTTGGACAAACCAC
sequencing_primer_5	TTTGCCTTTCTCTCCACA
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	CACNA1B
id	100008979

genbank_ids

mutation

W391A

name

cacna1b

shRNA_sequence

size

7797

species

9986

Oryctolagus cuniculus