This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cav2.2 BBS W391A pcDNA3
catalog :
206077
citations: 1
Reference
Cassidy J, Ferron L, Kadurin I, Pratt W, Dolphin A. Functional exofacially tagged N-type calcium channels elucidate the interaction with auxiliary α2δ-1 subunits. Proc Natl Acad Sci U S A. 2014;111:8979-84 pubmed publisher
product information
Catalog Number :
206077
Product Name :
Cav2.2 BBS W391A pcDNA3
article :
doi10.1073/pnas.1403731111
id28238446
pubmed_id24889613
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen Life Technologies
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
5' end of DNA is very GC rich
growth strain :
Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding
origin :
37
pi :
alt_names
Cav2.2 alpha1B
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5unknown
promoterCMV
sequencing_primer_3GCATTTAGGTGACACTATAG
sequencing_primer_5CTGGCTAACTAGAGAACC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCACNA1B
id100008979
genbank_ids
mutationW391A
namecacna1b
shRNA_sequence
size7178
species
9986
Oryctolagus cuniculus
tags
plasmid copy :
Yasuo Mori
resistance markers :
2017
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA