This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP Cav2.2 D122A pcDNA3
catalog :
206068
citations: 1
Reference
Page K, Heblich F, Davies A, Butcher A, Leroy J, Bertaso F, et al. Dominant-negative calcium channel suppression by truncated constructs involves a kinase implicated in the unfolded protein response. J Neurosci. 2004;24:5400-9 pubmed
product information
Catalog Number :
206068
Product Name :
GFP Cav2.2 D122A pcDNA3
article :
doi10.1523/JNEUROSCI.0553-04.2004
id28238452
pubmed_id15190113
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen Life Technologies
backbone_size5163
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
5' end of DNA is very GC rich
growth strain :
expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1
origin :
37
pi :
alt_names
Cav2.2 alpha1B
D122A
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5unknown
promoterCMV
sequencing_primer_3GCATTTAGGTGACACTATAG
sequencing_primer_5CTGGCTAACTAGAGAACC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCACNA1B
id100008979
genbank_ids
mutation
namecacna1b
shRNA_sequence
size7797
species
9986
Oryctolagus cuniculus
tags
locationN terminal on insert
tagGFP
plasmid copy :
Yasuo Mori
resistance markers :
2017
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA