pWZL Neo Myr Flag PRKAA1 | Addgene 20595 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pWZL Neo Myr Flag PRKAA1

catalog :

20595

citations: 4

Reference
Bakula D, Müller A, Zuleger T, Takacs Z, Franz Wachtel M, Thost A, et al. WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy. Nat Commun. 2017;8:15637 pubmed publisher
He M, Tan B, Vasan K, Yuan H, Cheng F, Ramos da Silva S, et al. SIRT1 and AMPK pathways are essential for the proliferation and survival of primary effusion lymphoma cells. J Pathol. 2017;242:309-321 pubmed publisher
Yen C, Chiang W, Liu S, Lin C, Liao K, Lin C, et al. Impacts of autophagy-inducing ingredient of areca nut on tumor cells. PLoS ONE. 2015;10:e0128011 pubmed publisher
Boehm J, Zhao J, Yao J, Kim S, Firestein R, Dunn I, et al. Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007;129:1065-79 pubmed

product information

Catalog Number :

20595

Product Name :

pWZL Neo Myr Flag PRKAA1

article :

doi	10.1016/j.cell.2007.03.052
id	2406
pubmed_id	17574021

bacterial resistance :

Ampicillin

cloning :

backbone

pWZL-Neo-Myr-Flag-DEST

backbone_mutation

backbone_origin

William Hahn Lab (available at Addgene, #15300)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 Addgene's sequencing results suggest that the best reference sequence for the PRKAA1 insert in this plasmid is AB022017.1.

growth temp :

Stbl3 cells to prevent recombination.

origin :

pi :

alt_names

protein kinase, AMP-activated, alpha 1 catalytic subunit

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	pWZL-Rev (TTC CGG GCC CTC ACA TTG)
sequencing_primer_5	pWZL-Fwd (GAA CCT CCT CGT TCG ACC)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AMPK, AMPK alpha 1, AMPKa1
gene	PRKAA1
id	5562

genbank_ids

mutation

name

PRKAA1

shRNA_sequence

size

species

9606

Homo sapiens