pWZL Neo Myr Flag PKM2 | Addgene 20585 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pWZL Neo Myr Flag PKM2

catalog :

20585

citations: 5

Reference
Wang C, Shyu R, Wu C, Chen M, Lee M, Lin Y, et al. Tazarotene-Induced Gene 1 Interacts with DNAJC8 and Regulates Glycolysis in Cervical Cancer Cells. Mol Cells. 2018;41:562-574 pubmed publisher
Wang H, Nicolay B, Chick J, Gao X, Geng Y, Ren H, et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature. 2017;546:426-430 pubmed publisher
Iansante V, Choy P, Fung S, Liu Y, Chai J, Dyson J, et al. PARP14 promotes the Warburg effect in hepatocellular carcinoma by inhibiting JNK1-dependent PKM2 phosphorylation and activation. Nat Commun. 2015;6:7882 pubmed publisher
Aeberhard L, Banhart S, Fischer M, Jehmlich N, Rose L, Koch S, et al. The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components. PLoS Pathog. 2015;11:e1004883 pubmed publisher
Boehm J, Zhao J, Yao J, Kim S, Firestein R, Dunn I, et al. Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007;129:1065-79 pubmed

product information

Catalog Number :

20585

Product Name :

pWZL Neo Myr Flag PKM2

article :

doi	10.1016/j.cell.2007.03.052
id	2406
pubmed_id	17574021

bacterial resistance :

Ampicillin

cloning :

backbone

pWZL-Neo-Myr-Flag-DEST

backbone_mutation

backbone_origin

William Hahn Lab (available at Addgene, #15300)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021

growth temp :

Stbl3 cells to prevent recombination.

origin :

pi :

alt_names

pyruvate kinase, muscle

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	pWZL-Rev (TTC CGG GCC CTC ACA TTG)
sequencing_primer_5	pWZL-Fwd (GAA CCT CCT CGT TCG ACC)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58
gene	PKM
id	5315

genbank_ids

NM_002654

mutation

name

PKM2

shRNA_sequence

size

species

9606

Homo sapiens