pGEMHE-RnCENP-O-298-5NS-GFP | Addgene 205742 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGEMHE-RnCENP-O-298-5NS-GFP

catalog :

205742

citations: 1

Reference
Dudka D, Akins R, Lampson M. FREEDA: An automated computational pipeline guides experimental testing of protein innovation. J Cell Biol. 2023;222: pubmed publisher

product information

Catalog Number :

205742

Product Name :

pGEMHE-RnCENP-O-298-5NS-GFP

article :

doi	10.1083/jcb.202212084
id	28238355
pubmed_id	37358475

bacterial resistance :

Ampicillin

cloning :

backbone

pGEMHE

backbone_mutation

backbone_origin

backbone_size

3043

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

In vitro transcription

growth strain :

Expresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain and 5 mouse-specific point mutations; C-term tagged with GFP under T7 promoter - designed for in vitro transcription

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	T7
sequencing_primer_3
sequencing_primer_5	CCTCTTCGCTATTACGCCAG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	2810429O05Rik, 8430427C03Rik, D12Ertd482e
gene	Cenpo
id	52504

aliases
gene	Cenpo
id	684439

genbank_ids

NC_000078.7 (mouse)

NC_051341.1 (rat)

mutation

Chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain and 5 mouse-specific point mutations in residues under positive selection

name

CENP-O

shRNA_sequence

size

1629

species

10090

Mus musculus

10116

Rattus norvegicus