This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1(+)-RasAR-NES
catalog :
205567
citations: 1
Reference
Weeks R, Zhou X, Yuan T, Zhang J. Fluorescent Biosensor for Measuring Ras Activity in Living Cells. J Am Chem Soc. 2022;144:17432-17440 pubmed publisher
product information
Catalog Number :
205567
Product Name :
pcDNA3.1(+)-RasAR-NES
article :
doi10.1021/jacs.2c05203
id28234226
pubmed_id36122391
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5363
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.
origin :
37
pi :
alt_names
Cerulean3-RBD(Raf1 51-131)-pseudoligand-YPet-NES
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMD1NN, CRAF, NS5, Raf-1, c-Raf
geneRAF1
id5894
genbank_ids
mutation
nameRasAR-NES
shRNA_sequence
size1788
species
9606
Homo sapiens
100
Synthetic
tags
locationC terminal on insert
tagNuclear export signal (NES)
resistance markers :
2182
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA