This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pWZL Neo Myr Flag MAP3K7
catalog :
20520
citations: 1
| Reference |
|---|
Boehm J, Zhao J, Yao J, Kim S, Firestein R, Dunn I, et al. Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007;129:1065-79 pubmed
|
product information
Catalog Number :
20520
Product Name :
pWZL Neo Myr Flag MAP3K7
article :
| doi | 10.1016/j.cell.2007.03.052 |
| id | 2406 |
| pubmed_id | 17574021 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pWZL-Neo-Myr-Flag-DEST | ||
| backbone_mutation | |||
| backbone_origin | William Hahn Lab (available at Addgene, #15300) | ||
| backbone_size | |||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Addgene has identified a Q356H mutation in the MAP3K7 insert. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
growth temp :
Stbl3 cells to prevent recombination.
origin :
37
pi :
|
plasmid copy :
1-G8
resistance markers :
| 184 |
| 613 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments