This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEMHE-RnCENP-O-80-GFP
catalog :
205182
citations: 1
Reference
Dudka D, Akins R, Lampson M. FREEDA: An automated computational pipeline guides experimental testing of protein innovation. J Cell Biol. 2023;222: pubmed publisher
product information
Catalog Number :
205182
Product Name :
pGEMHE-RnCENP-O-80-GFP
article :
doi10.1083/jcb.202212084
id28238355
pubmed_id37358475
bacterial resistance :
Ampicillin
cloning :
backbonepGEMHE
backbone_mutation
backbone_origin
backbone_size3043
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
In vitro transcription
growth strain :
Expresses chimeric rat CENP-O with mouse CENP-O N-terminus; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3
sequencing_primer_5CCTCTTCGCTATTACGCCAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases2810429O05Rik, 8430427C03Rik, D12Ertd482e
geneCenpo
id52504
aliases
geneCenpo
id684439
genbank_ids
NC_000078.7 (mouse)
NC_051341.1 (rat)
mutationChimeric rat CENP-O with mouse CENP-O N terminal region
nameCENP-O
shRNA_sequence
size1632
species
10090
Mus musculus
10116
Rattus norvegicus
tags
locationC terminal on insert
tag5 glycines linker followed by GFP
resistance markers :
1550
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA