pWZL Neo Myr Flag FGFR1 | Addgene 20486 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pWZL Neo Myr Flag FGFR1

catalog :

20486

citations: 9

Reference
Fang Q, Xin W, Chen L, Fu Y, Qi Y, Ding H, et al. Caffeic acid phenethyl ester suppresses metastasis of breast cancer cells by inactivating FGFR1 via MD2. PLoS ONE. 2023;18:e0289031 pubmed publisher
Vang A, da Silva Gonçalves Bós D, Fernandez Nicolas A, Zhang P, Morrison A, Mancini T, et al. α7 Nicotinic acetylcholine receptor mediates right ventricular fibrosis and diastolic dysfunction in pulmonary hypertension. JCI Insight. 2021;6: pubmed publisher
Mouron S, Manso L, Caleiras E, Rodriguez Peralto J, Rueda O, Caldas C, et al. FGFR1 amplification or overexpression and hormonal resistance in luminal breast cancer: rationale for a triple blockade of ER, CDK4/6, and FGFR1. Breast Cancer Res. 2021;23:21 pubmed publisher
Shi Y, Ma Z, Cheng Q, Wu Y, Parris A, Kong L, et al. FGFR1 overexpression renders breast cancer cells resistant to metformin through activation of IRS1/ERK signaling. Biochim Biophys Acta Mol Cell Res. 2021;1868:118877 pubmed publisher
Andriani F, Majorini M, Mano M, Landoni E, Miceli R, Facchinetti F, et al. MiR-16 regulates the pro-tumorigenic potential of lung fibroblasts through the inhibition of HGF production in an FGFR-1- and MEK1-dependent manner. J Hematol Oncol. 2018;11:45 pubmed publisher
Hopkins A, Coatham M, Berry F. FOXC1 Regulates FGFR1 Isoform Switching to Promote Invasion Following TGF?-Induced EMT. Mol Cancer Res. 2017;15:1341-1353 pubmed publisher
Knelson E, Gaviglio A, Nee J, Starr M, Nixon A, Marcus S, et al. Stromal heparan sulfate differentiates neuroblasts to suppress neuroblastoma growth. J Clin Invest. 2014;124:3016-31 pubmed publisher
Knelson E, Gaviglio A, Tewari A, Armstrong M, Mythreye K, Blobe G. Type III TGF-β receptor promotes FGF2-mediated neuronal differentiation in neuroblastoma. J Clin Invest. 2013;123:4786-98 pubmed
Boehm J, Zhao J, Yao J, Kim S, Firestein R, Dunn I, et al. Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007;129:1065-79 pubmed

product information

Catalog Number :

20486

Product Name :

pWZL Neo Myr Flag FGFR1

article :

doi	10.1016/j.cell.2007.03.052
id	2406
pubmed_id	17574021

bacterial resistance :

Ampicillin

cloning :

backbone

pWZL-Neo-Myr-Flag-DEST

backbone_mutation

backbone_origin

William Hahn Lab (available at Addgene, #15300)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021

growth temp :

Stbl3 cells to prevent recombination.

origin :

pi :

alt_names

fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome)

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	pWZL-Rev (TTC CGG GCC CTC ACA TTG)
sequencing_primer_5	pWZL-Fwd (GAA CCT CCT CGT TCG ACC)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1
gene	FGFR1
id	2260

genbank_ids

NM_000604

mutation

name

FGFR1

shRNA_sequence

size

species

9606

Homo sapiens