This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pR008_APAD
catalog :
204818
citations: 1
Reference
Ruan X, Hu K, Zhang X. PIE-seq: identifying RNA-binding protein targets by dual RNA-deaminase editing and sequencing. Nat Commun. 2023;14:3275 pubmed publisher
product information
Catalog Number :
204818
Product Name :
pR008_APAD
article :
doi10.1038/s41467-023-39054-8
id28238279
pubmed_id37280234
bacterial resistance :
Ampicillin
cloning :
backbonepCAGIG
backbone_mutation
backbone_originConnie Cepko Lab
backbone_size6124
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression Apobec1-ADAR2dd fusion protein as PIE-RBP control
origin :
37
pi :
alt_names
Apobec1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterchicken -actin promoter
sequencing_primer_3GGGGGCGGAATTTACGTAGC
sequencing_primer_5GCTAACCATGTTCATGCCTTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesREPR, apobec-1
geneApobec1
id25383
genbank_ids
NM_012907
mutation
namerat Apobec1 and human ADAR2 deaminase domain with engineered sites
shRNA_sequence
size1950
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagHA and Flag
locationC terminal on insert
taghuADAR2dd
plasmid copy :
rApobec1-XTEN was amplified from pCMV-BE3 (Addgene #73021), and hADAR2dd was amplified from pC0055 (Addgene #103871) .
resistance markers :
6585
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA