This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMZ3012
catalog :
204400
citations: 1
Reference
Zeng M, Sarker B, Howitz N, Shah I, Andrews L. Synthetic Homoserine Lactone Sensors for Gram-Positive Bacillus subtilis Using LuxR-Type Regulators. ACS Synth Biol. 2023;: pubmed publisher
product information
Catalog Number :
204400
Product Name :
pMZ3012
article :
doi10.1021/acssynbio.3c00504
id28238224
pubmed_id38079538
bacterial resistance :
Kanamycin
cloning :
backbonepNH4
backbone_mutationThis is a shuttle vector containing AmyE homologous arms for integration into B. subtilis genome.
backbone_origin
backbone_size6429
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Synthetic Biology
growth strain :
A C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.
growth temp :
chloramphenicol ( 5 g/ml) resistant when integrated to B. subtilis genome
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterPftsH
sequencing_primer_3AGATCTTCATCACCGAAACGC
sequencing_primer_5NULL
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesPA3477
generhlR
id878968
genbank_ids
mutation
namequorum sensing regulator rhlR
shRNA_sequence
size720
species
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterSynthetic promoter PRhl0L0L12
sequencing_primer_3
sequencing_primer_5GACAAATCCGCCGCTCTAG
site_3_destroyed1
site_5_destroyed1
entrez_gene
genbank_ids
mutation
nameGFP
shRNA_sequence
size717
species
tags
resistance markers :
5271
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA