This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AAVS1-Hb9-CD14
catalog :
204344
product information
Catalog Number :
204344
Product Name :
AAVS1-Hb9-CD14
article :
doi
id28238214
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backboneAAVS1-CAG-hrGFP
backbone_mutationThe Not1 site in the backbone was deleted, and the CAG-hrGFP sequence was removed. The original insert was replaced by the 9kb Hb9 promoter (PMID: 10482234), a 5' splice substrate (PMID: 2038318), the human CD14 gene (PMID: 24496616) and the bGH-poly-A signal (PMID: 6146135). This entire cassette can be excised by Not1/Pac1 digest.
backbone_originSu-Chun Zhang - plasmid# 52344
backbone_size7100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Spe1
cloning_site_5Age1
promotermouse Hb9 (Mnx1) 9kb promoter fragment
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5TGGAAGGTGCCACTCCCACTGT
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCD14
id929
genbank_ids
NM_000591
mutation
namehuman CD14
shRNA_sequence
size1159
species
9606
Homo sapiens
tags
resistance markers :
2068
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA