This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pWZL Neo Myr Flag BTK
catalog :
20432
citations: 3
Reference
Umezawa Y, Akiyama H, Okada K, Ishida S, Nogami A, Oshikawa G, et al. Molecular mechanisms for enhancement of stromal cell-derived factor 1-induced chemotaxis by platelet endothelial cell adhesion molecule 1 (PECAM-1). J Biol Chem. 2017;292:19639-19655 pubmed publisher
Gurung P, Fan G, Lukens J, Vogel P, Tonks N, Kanneganti T. Tyrosine Kinase SYK Licenses MyD88 Adaptor Protein to Instigate IL-1?-Mediated Inflammatory Disease. Immunity. 2017;46:635-648 pubmed publisher
Boehm J, Zhao J, Yao J, Kim S, Firestein R, Dunn I, et al. Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007;129:1065-79 pubmed
product information
Catalog Number :
20432
Product Name :
pWZL Neo Myr Flag BTK
article :
doi10.1016/j.cell.2007.03.052
id2406
pubmed_id17574021
bacterial resistance :
Ampicillin
cloning :
backbonepWZL-Neo-Myr-Flag-DEST
backbone_mutation
backbone_originWilliam Hahn Lab (available at Addgene, #15300)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
growth temp :
Stbl3 cells to prevent recombination.
origin :
37
pi :
alt_names
Bruton agammaglobulinemia tyrosine kinase
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3pWZL-Rev (TTC CGG GCC CTC ACA TTG)
sequencing_primer_5pWZL-Fwd (GAA CCT CCT CGT TCG ACC)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAGMX1, AT, ATK, BPK, IGHD3, IMD1, PSCTK1, XLA
geneBTK
id695
genbank_ids
NM_000061
mutation
nameBTK
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagMyr
locationN terminal on backbone
tagFlag
plasmid copy :
2-E8
resistance markers :
184
613
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA