This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pWZL Neo Myr Flag BLK
catalog :
20430
citations: 2
| Reference |
|---|
Compeer E, Janssen W, van Royen Kerkhof A, van Gijn M, van Montfrans J, Boes M. Dysfunctional BLK in common variable immunodeficiency perturbs B-cell proliferation and ability to elicit antigen-specific CD4+ T-cell help. Oncotarget. 2015;6:10759-71 pubmed
|
Boehm J, Zhao J, Yao J, Kim S, Firestein R, Dunn I, et al. Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007;129:1065-79 pubmed
|
product information
Catalog Number :
20430
Product Name :
pWZL Neo Myr Flag BLK
article :
| doi | 10.1016/j.cell.2007.03.052 |
| id | 2406 |
| pubmed_id | 17574021 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pWZL-Neo-Myr-Flag-DEST | ||
| backbone_mutation | |||
| backbone_origin | William Hahn Lab (available at Addgene, #15300) | ||
| backbone_size | |||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
growth temp :
Stbl3 cells to prevent recombination.
origin :
37
pi :
|
plasmid copy :
1-G7
resistance markers :
| 184 |
| 613 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments