pWZL Neo Myr Flag AXL | Addgene 20428 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pWZL Neo Myr Flag AXL

catalog :

20428

citations: 5

Reference
Pietrobono S, Anichini G, Sala C, Manetti F, Almada L, Pepe S, et al. ST3GAL1 is a target of the SOX2-GLI1 transcriptional complex and promotes melanoma metastasis through AXL. Nat Commun. 2020;11:5865 pubmed publisher
Hoj J, Mayro B, Pendergast A. A TAZ-AXL-ABL2 Feed-Forward Signaling Axis Promotes Lung Adenocarcinoma Brain Metastasis. Cell Rep. 2019;29:3421-3434.e8 pubmed publisher
Brenig R, Pop O, Triantafyllou E, Geng A, Singanayagam A, Perez Shibayama C, et al. Expression of AXL receptor tyrosine kinase relates to monocyte dysfunction and severity of cirrhosis. Life Sci Alliance. 2020;3: pubmed publisher
Wu D, Wang Y, Wang L, Chen C, Lee H. A low microRNA-630 expression confers resistance to tyrosine kinase inhibitors in EGFR-mutated lung adenocarcinomas via miR-630/YAP1/ERK feedback loop. Theranostics. 2018;8:1256-1269 pubmed publisher
Boehm J, Zhao J, Yao J, Kim S, Firestein R, Dunn I, et al. Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell. 2007;129:1065-79 pubmed

product information

Catalog Number :

20428

Product Name :

pWZL Neo Myr Flag AXL

article :

doi	10.1016/j.cell.2007.03.052
id	2406
pubmed_id	17574021

bacterial resistance :

Ampicillin

cloning :

backbone

pWZL-Neo-Myr-Flag-DEST

backbone_mutation

backbone_origin

William Hahn Lab (available at Addgene, #15300)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021

growth temp :

Stbl3 cells to prevent recombination.

origin :

pi :

alt_names

AXL receptor tyrosine kinase

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	pWZL-Rev (TTC CGG GCC CTC ACA TTG)
sequencing_primer_5	pWZL-Fwd (GAA CCT CCT CGT TCG ACC)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ARK, AXL3, JTK11, Tyro7, UFO
gene	AXL
id	558

genbank_ids

BC032229.1

AAH32229.1

mutation

name

AXL

shRNA_sequence

size

species

9606

Homo sapiens