mEos3.2GG | Addgene 203756 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

mEos3.2GG

catalog :

203756

citations: 1

Reference
Shelby S, Castelló Serrano I, Wisser K, Levental I, Veatch S. Membrane phase separation drives responsive assembly of receptor signaling domains. Nat Chem Biol. 2023;19:750-758 pubmed publisher

product information

Catalog Number :

203756

Product Name :

mEos3.2GG

article :

doi	10.1038/s41589-023-01268-8
id	28238144
pubmed_id	36997644

bacterial resistance :

Kanamycin

cloning :

backbone

mEos3.2-C1

backbone_mutation

backbone_origin

Addgene; Michael Davidson's lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5	Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
gene	KRAS
id	3845

genbank_ids

mutation

name

KRas c-term

shRNA_sequence

size

species

9606

Homo sapiens