This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
psicheck2-MAIP1mut
catalog :
203604
citations: 1
Reference
Sakai E, Imaizumi T, Suzuki R, Taracena G xe1 ndara M, Fujimoto T, Sakurai F, et al. miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells. Commun Biol. 2023;6:669 pubmed publisher
product information
Catalog Number :
203604
Product Name :
psicheck2-MAIP1mut
article :
doi10.1038/s42003-023-05049-w
id28238117
pubmed_id37355744
bacterial resistance :
Ampicillin
cloning :
backbonepsicheck2
backbone_mutationThe 3'UTR (nt 1065-1235) of MAIP1 with mutated miR-27b target sequences was inserted between XhoI and NotI in psicheck2.
backbone_originPromega
backbone_size6273
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth strain :
Reporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequences
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XhoI
promoterSV40
sequencing_primer_3ccgcgaggtccgaagactc
sequencing_primer_5gtggagcgcgtgctgaagaac
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC2orf47
geneMAIP1
id79568
genbank_ids
NM_001369399.1
mutationmiR-27b target sequences in the 3'UTR was mutated
namematrix AAA peptidase interacting protein 1
shRNA_sequence
size171
species
9606
Homo sapiens
tags
resistance markers :
6545
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA