This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
psicheck2-27bT
catalog :
203600
citations: 1
Reference
Sakai E, Imaizumi T, Suzuki R, Taracena G xe1 ndara M, Fujimoto T, Sakurai F, et al. miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells. Commun Biol. 2023;6:669 pubmed publisher
product information
Catalog Number :
203600
Product Name :
psicheck2-27bT
article :
doi10.1038/s42003-023-05049-w
id28238117
pubmed_id37355744
bacterial resistance :
Ampicillin
cloning :
backbonepsicheck2
backbone_mutationThe complementary sequence to human miR-27b-3p was inserted between XhoI and NotI in psicheck2
backbone_originPromega
backbone_size6273
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth strain :
Reporter plasmid carrying miR-27b target sequence
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XhoI
promoterSV40
sequencing_primer_3ccgcgaggtccgaagactc
sequencing_primer_5gtggagcgcgtgctgaagaac
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIR-27b, MIRN27B, miRNA27B
geneMIR27B
id407019
genbank_ids
MIMAT0000419
mutation
namecomplementary sequence to miR27b-3p
shRNA_sequence
size21
species
9606
Homo sapiens
tags
resistance markers :
6545
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA