This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PT3.5/CMV-EGFRvIII
catalog :
20280
citations: 4
Reference
Ando S, Kojima N, Moyama C, Fujita M, Ohta K, Ii H, et al. JCI‑20679 suppresses the proliferation of glioblastoma stem cells by activating AMPK and decreasing NFATc2 expression levels. Mol Med Rep. 2022;26: pubmed publisher
Tuzmen C, Cairns T, Atanasiu D, Lou H, Saw W, Hall B, et al. Point Mutations in Retargeted gD Eliminate the Sensitivity of EGFR/EGFRvIII-Targeted HSV to Key Neutralizing Antibodies. Mol Ther Methods Clin Dev. 2020;16:145-154 pubmed publisher
Suzuki S, Yamamoto M, Sanomachi T, Togashi K, Sugai A, Seino S, et al. Brexpiprazole, a Serotonin-Dopamine Activity Modulator, Can Sensitize Glioma Stem Cells to Osimertinib, a Third-Generation EGFR-TKI, via Survivin Reduction. Cancers (Basel). 2019;11: pubmed publisher
Wiesner S, Decker S, Larson J, Ericson K, Forster C, Gallardo J, et al. De novo induction of genetically engineered brain tumors in mice using plasmid DNA. Cancer Res. 2009;69:431-9 pubmed publisher
product information
Catalog Number :
20280
Product Name :
PT3.5/CMV-EGFRvIII
article :
doi10.1158/0008-5472.CAN-08-1800
id2433
pubmed_id19147555
bacterial resistance :
Ampicillin
cloning :
backbonecolE1/ampR
backbone_mutation
backbone_originOhlfest Lab
backbone_size5481
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Transposon
growth notes :
Addgene NGS results identified a E423G variant in EGFRvIII compared to the NCBI reference (NP_001333870.1). We do not know if this variant affects function.
growth temp :
DH5
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA
geneEGFR
id1956
genbank_ids
mutationSee depositor comments below.
nameEGFRvII
shRNA_sequence
size2832
species
9606
Homo sapiens
tags
resistance markers :
367
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA