This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGCNK58
catalog :
202461
product information
Catalog Number :
202461
Product Name :
pGCNK58
article :
| doi | 10.1038/347575a0 |
| id | 28237981 |
| pubmed_id | 2145515 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pet3b | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
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growth notes :
Dynamics of GCN4 facilitate DNA interaction: a model-free analysis of an intrinsically disordered region. Gill ML, Byrd RA, Palmer AG III. Phys Chem Chem Phys. 2016 Feb 17;18(8):5839-49. doi: 10.1039/c5cp06197k. The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted a-helices: crystal structure of the protein-DNA complex. Ellenberger, T. E., Brandl, C. J., Struhl, K. & Harrison, S. C. (1992). Cell, 71, 1223-1237. PMID 1473154.
growth strain :
Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells
origin :
37
pi :
AAS101, AAS3, ARG9gene GCN4 id 856709 genbank_ids mutation name Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension shRNA_sequence size 177 species
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