This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRmHa3-dKDM4A-H195A-HA2FL2
catalog :
20235
citations: 1
Reference
Lin C, Li B, Swanson S, Zhang Y, Florens L, Washburn M, et al. Heterochromatin protein 1a stimulates histone H3 lysine 36 demethylation by the Drosophila KDM4A demethylase. Mol Cell. 2008;32:696-706 pubmed publisher
product information
Catalog Number :
20235
Product Name :
pRmHa3-dKDM4A-H195A-HA2FL2
article :
doi10.1016/j.molcel.2008.11.008
id2436
pubmed_id19061644
bacterial resistance :
Ampicillin
cloning :
backbonepRmHa3-CHA2FL2
backbone_mutation
backbone_origin
backbone_size3990
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
D153E mutation is also present, but it does affect the plasmid.
origin :
37
pi :
alt_names
CG15835
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5KpnI
promoter
sequencing_primer_3gtagagcgtttatcagttttttgg
sequencing_primer_5agaggtgaatcgaacgaaagacccg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCG15835, dJMJD2(1), dKDM4A, Dmel CG15835
geneKdm4A
id35744
genbank_ids
NM_136487
mutationchanged His 195 to Ala
namedKDM4A
shRNA_sequence
size1488
species
7227
Drosophila melanogaster
tags
locationC terminal on backbone
tagHA2
locationC terminal on backbone
tagFLAG2
resistance markers :
588
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA