pBacPAK-HFT-dKDM4B | Addgene 20234 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBacPAK-HFT-dKDM4B

catalog :

20234

citations: 1

Reference
Lin C, Li B, Swanson S, Zhang Y, Florens L, Washburn M, et al. Heterochromatin protein 1a stimulates histone H3 lysine 36 demethylation by the Drosophila KDM4A demethylase. Mol Cell. 2008;32:696-706 pubmed publisher

product information

Catalog Number :

20234

Product Name :

pBacPAK-HFT-dKDM4B

article :

doi	10.1016/j.molcel.2008.11.008
id	2436
pubmed_id	19061644

bacterial resistance :

Ampicillin

cloning :

backbone

pBacPAK-HFT

backbone_mutation

backbone_origin

backbone_size

5608

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

TEV tag ENPYFQG instead of ENLYFQG. The cDNA used in making dKDM4B(CG33182) constructs has incomplete 3' end. It is because NCBI data has updated the sequence after I made the construct. There is a comment on NCBI page https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=161077032&from=148&to=1920&view=gbwithparts indicates that they replaced a former sequence of CG33182 https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=28573833 with the current 1773bp one.

origin :

pi :

alt_names

CG33182

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	XhoI
promoter
sequencing_primer_3	ACGCACAGAATCTAGCGCT
sequencing_primer_5	ccattgtaatgagacgca
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG33182, CG33182, CG4037, Dmel CG33182, KDM4B, dJMJD2(2), dKDM4B, dKdm4B
gene	Kdm4B
id	318918

genbank_ids

NM_176164

mutation

name

dKDM4B

shRNA_sequence

size

1359

species

7227

Drosophila melanogaster