This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Vh-HA-2xFKBP
catalog :
20221
citations: 1
Reference
Czlapinski J, Schelle M, Miller L, Laughlin S, Kohler J, Cornish V, et al. Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. J Am Chem Soc. 2008;130:13186-7 pubmed publisher
product information
Catalog Number :
20221
Product Name :
Vh-HA-2xFKBP
article :
doi10.1021/ja8037728
id2435
pubmed_id18788807
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
murine VH chain signal peptide followed by a HA tag and two repeats of FKBP
origin :
37
pi :
alt_names
FKBP
FK506 binding protein
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5NdeI
promoter
sequencing_primer_3BGH reverse
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE
geneFKBP1A
id2280
genbank_ids
mutation
name2xFKBP
shRNA_sequence
size879
species
9606
Homo sapiens
tags
locationN terminal on insert
tagVh
locationN terminal on insert
tagHA
plasmid copy :
FKBP gene was a gift from David Austin & Fei Liu (Yale University)
resistance markers :
32
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA