This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
DHFR-myc
catalog :
20214
citations: 5
Reference
Meurant S, Mauclet L, Dieu M, Arnould T, Eyckerman S, Renard P. Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells. Int J Mol Sci. 2023;24: pubmed publisher
Gutnick A, Banghart M, West E, Schwarz T. The light-sensitive dimerizer zapalog reveals distinct modes of immobilization for axonal mitochondria. Nat Cell Biol. 2019;: pubmed publisher
Lee J, Xu Y, Zhang T, Cui L, Saidi L, Ye Y. Secretion of misfolded cytosolic proteins from mammalian cells is independent of chaperone-mediated autophagy. J Biol Chem. 2018;293:14359-14370 pubmed publisher
Jing C, Cornish V. Design, synthesis, and application of the trimethoprim-based chemical tag for live-cell imaging. Curr Protoc Chem Biol. 2013;5:131-55 pubmed publisher
Czlapinski J, Schelle M, Miller L, Laughlin S, Kohler J, Cornish V, et al. Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. J Am Chem Soc. 2008;130:13186-7 pubmed publisher
product information
Catalog Number :
20214
Product Name :
DHFR-myc
article :
doi10.1021/ja8037728
id2435
pubmed_id18788807
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1/Zeo(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
dihydrofolate reductase
folA
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5XbaI
promoter
sequencing_primer_3BGH reverse
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0048, ECK0049, tmr, tmrA
genefolA
id944790
genbank_ids
mutation
nameDHFR
shRNA_sequence
size507
species
E. coli
tags
locationC terminal on insert
tagmyc
resistance markers :
32
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA