This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMC-TNFalpha
catalog :
20210
citations: 1
Reference
Zhao T, Graber T, Jordan L, Cloutier M, Lewis S, Goulet I, et al. hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Cell Death Differ. 2009;16:244-52 pubmed publisher
product information
Catalog Number :
20210
Product Name :
pMC-TNFalpha
article :
doi10.1038/cdd.2008.146
id2434
pubmed_id18846111
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutationThe expression plasmid pMC was constructed by inserting a synthetic polylinker (5 -AGC TTG CGG CCG CTA GCC TCG AGG GAT CCT CTA GAA TGC A-3 ) into a pcDNA3 vector (Invitrogen) followed by insertion of the chloramphenicol acetyl transferase (CAT) coding sequence. The CAT insert was generated by PCR amplification using pCAT as a template.
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The 3 UTR was generated by PCR amplification from a liver cDNA library (Invitrogen). and the expression plasmids pMC-TNF was constructed by inserting the 3 UTR of TNF downstream of CAT in the pMC construct.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3
sequencing_primer_5CAT-F (5'-GTTTGTGATGGCTTCCATGTC-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDIF, TNF-alpha, TNFA, TNFSF2, TNLG1F
geneTNF
id7124
genbank_ids
mutation
nameTNFa 3' UTR
shRNA_sequence
size808
species
9606
Homo sapiens
tags
resistance markers :
206
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA