This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
1BR9-AtWUSSTOP- 3
catalog :
202056
citations: 1
Reference
Wang J, Squire H, Goh N, Ni H, Lien E, Wong C, et al. Delivered complementation in planta (DCIP) enables measurement of peptide-mediated protein delivery efficiency in plants. Commun Biol. 2023;6:840 pubmed publisher
product information
Catalog Number :
202056
Product Name :
1BR9-AtWUSSTOP- 3
article :
doi10.1038/s42003-023-05191-5
id28233199
pubmed_id37573467
bacterial resistance :
Kanamycin
cloning :
backbone1BR9
backbone_mutation
backbone_origin
backbone_size5513
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2022.05.03.490515v2 for bioRxiv preprint.
growth strain :
E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7 Promoter
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G17950, PGA6, T27K22.18, T27K22_18, WUS1, WUSCHEL, WUSCHEL 1
geneWUS
id816305
genbank_ids
NM_127349
mutationthird helix of homeodomain deleted (AA82-102)
nameAtWUS E. Coli Codon Optimized, third helix of homeodomain deleted
shRNA_sequence
size810
species
3702
Arabidopsis thaliana
tags
locationN terminal on backbone
tag6xHis
locationN terminal on backbone
tagGFP11
resistance markers :
5489
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA