This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRBUBR1
catalog :
20198
citations: 1
Reference
Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A. Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end rule pathway. J Biol Chem. 2008;283:24011-28 pubmed publisher
product information
Catalog Number :
20198
Product Name :
pRBUBR1
article :
doi10.1074/jbc.M802583200
id2427
pubmed_id18566452
bacterial resistance :
Ampicillin
cloning :
backboneYeplac 181
backbone_mutation
backbone_origin
backbone_size5741
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Plasmid for overexpressing Ubr1ha in yeast. The ha tag is at the C-terminus of the protein and the protein is active in degrading N-end rule substrates.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promoter
sequencing_primer_3M13-puc-Rev
sequencing_primer_5yADH1-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYGR184C, PTR1
geneUBR1
id853096
genbank_ids
mutation
nameUBR1
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagHA
resistance markers :
545
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA