This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
3XFlagNICD3
catalog :
20185
citations: 4
Reference
Gazdik T, Crow J, Lawton T, Munroe C, Theriault H, Wood T, et al. Notch intracellular domains form transcriptionally active heterodimeric complexes on sequence-paired sites. Sci Rep. 2024;14:218 pubmed publisher
Crow J, Albig A. Notch family members follow stringent requirements for intracellular domain dimerization at sequence-paired sites. PLoS ONE. 2020;15:e0234101 pubmed publisher
Zhang C, Chen D, Margaret Maguire E, He S, Chen J, An W, et al. Cbx3 Inhibits Vascular Smooth Muscle Cell Proliferation, Migration and Neointima Formation. Cardiovasc Res. 2017;: pubmed publisher
Ong C, Cheng H, Chang L, Ohtsuka T, Kageyama R, Stormo G, et al. Target selectivity of vertebrate notch proteins. Collaboration between discrete domains and CSL-binding site architecture determines activation probability. J Biol Chem. 2006;281:5106-19 pubmed
product information
Catalog Number :
20185
Product Name :
3XFlagNICD3
article :
doi10.1074/jbc.M506108200
id2425
pubmed_id16365048
bacterial resistance :
Ampicillin
cloning :
backbonep3XFLAG-CMV-7
backbone_mutation
backbone_originSigma
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD3 was cloned into XbaI/BamHI sites. NICD3 (Met-1664 to Ala-2318 of full-length mouse N3) was amplified from the N3 plasmid (gift from Dr. U. Lendahl) with 5XbaN3 (5'-GCTCTAGAATGGTTGCCAGGCGAAAGCGAG-3') and 3BamN3 (5'-GCGGATCCCTAGGCCATCACCTGCCTCTTGGG-3') primers.
origin :
37
pi :
alt_names
NICD3
Notch 3
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5Xba1
promoterCMV
sequencing_primer_3hGH-pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesN3, hpbk
geneNotch3
id18131
genbank_ids
NM_008716.2
NP_032742.1
mutationContains murine Notch 3 Intracellular Domain (Met-1664 to Ala-2318)
nameNotch 3 Intracellular Domain
shRNA_sequence
size1962
species
10090
Mus musculus
tags
locationN terminal on backbone
tag3X Flag
plasmid copy :
Notch 3 plasmid from Dr. Urban Lendahl (Karolinska Institutet, Stockholm, Sweden).
resistance markers :
1405
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA