This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
YFP-FKBP (YF)
catalog :
20175
citations: 4
Reference
Besson B, Eun H, Kim S, Windisch M, Bourhy H, Grailhe R. Optimization of BRET saturation assays for robust and sensitive cytosolic protein-protein interaction studies. Sci Rep. 2022;12:9987 pubmed publisher
Miyamoto T, Uosaki H, Mizunoe Y, Han S, Goto S, Yamanaka D, et al. Rapid manipulation of mitochondrial morphology in a living cell with iCMM. Cell Rep Methods. 2021;1:100052 pubmed publisher
Song Z, Bouchab L, Hudik E, Le Bars R, NĂ¼sse O, Dupre Crochet S. Phosphoinositol 3-phosphate acts as a timer for reactive oxygen species production in the phagosome. J Leukoc Biol. 2017;101:1155-1168 pubmed publisher
Inoue T, Heo W, Grimley J, Wandless T, Meyer T. An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways. Nat Methods. 2005;2:415-8 pubmed
product information
Catalog Number :
20175
Product Name :
YFP-FKBP (YF)
article :
doi10.1038/nmeth763
id2411
pubmed_id15908919
bacterial resistance :
Kanamycin
cloning :
backboneEYFP-C1
backbone_mutation
backbone_originClontech
backbone_size4733
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
FKBP12
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho1
cloning_site_5BsrG1
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE
geneFKBP1A
id2280
genbank_ids
mutationFKBP was taken out from pC4M-F2E with a flanking sequence encoding BsrGI and XhoI, along with a flexible linker sequence (agt gct ggt ggt agt gct ggt ggt agt gct ggt ggt agt gct ggt ggt agt gct ggt ggt) at the C terminus of FKBP, and then inserted into the YFP (C1) vector.
nameYFP-FKBP (YF):
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
336
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA