pBS-Squ5'3' | Addgene 20165 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBS-Squ5'3'

catalog :

20165

citations: 1

Reference
Martin A, Kaschube M, Wieschaus E. Pulsed contractions of an actin-myosin network drive apical constriction. Nature. 2009;457:495-9 pubmed publisher

product information

Catalog Number :

20165

Product Name :

pBS-Squ5'3'

article :

doi	10.1038/nature07522
id	2392
pubmed_id	19029882

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript

backbone_mutation

backbone_origin

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

There are several polymorphisms in the 3 UTR of the expression construct and should not affect the function.

origin :

pi :

alt_names

sqh spaghetti squash

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	KpnI
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG3595, CG3595, DmMRLC_C, Dmel CG3595, MLC, MLRC, MRLC, Myo, Myo II, Myo-II, Myo-II RLC, MyoII, Myosin II, NMM, RLC, RLC2, RMLC, Sqh, actomyosin, myo II, myoII, myosin, myosin 2, myosin II, pMyo-II, sgh, spg, squ, ssq
gene	sqh
id	31554

genbank_ids

mutation

name

spaghetti squash (squ, myosin II RLC) 5' UTR, ORF (without stop codon) and 3' UTR with multiple cloning site downstream

shRNA_sequence

size

2799

species

7227

Drosophila melanogaster