This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS-Squ:mCherry
catalog :
20163
citations: 2
Reference
Lin B, Luo J, Lehmann R. An AMPK phosphoregulated RhoGEF feedback loop tunes cortical flow-driven amoeboid migration in vivo. Sci Adv. 2022;8:eabo0323 pubmed publisher
Martin A, Kaschube M, Wieschaus E. Pulsed contractions of an actin-myosin network drive apical constriction. Nature. 2009;457:495-9 pubmed publisher
product information
Catalog Number :
20163
Product Name :
pBS-Squ:mCherry
article :
doi10.1038/nature07522
id2392
pubmed_id19029882
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript
backbone_mutation
backbone_origin
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
There are several polymorphisms in the 3 UTR of the expression construct and should not affect the function.
growth temp :
DH5alpha
origin :
37
pi :
alt_names
sqh spaghetti squash
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5KpnI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG3595, CG3595, DmMRLC_C, Dmel CG3595, MLC, MLRC, MRLC, Myo, Myo II, Myo-II, Myo-II RLC, MyoII, Myosin II, NMM, RLC, RLC2, RMLC, Sqh, actomyosin, myo II, myoII, myosin, myosin 2, myosin II, pMyo-II, sgh, spg, squ, ssq
genesqh
id31554
genbank_ids
mutation
namespaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR
shRNA_sequence
size3503
species
7227
Drosophila melanogaster
tags
resistance markers :
581
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA