This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-6P-1_WT RAD23A
catalog :
201456
citations: 1
Reference
Ashton N, Jaiswal N, Moreno N, Semenova I, D Orlando D, Latância M, et al. A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases. J Mol Biol. 2023;435:168353 pubmed publisher
product information
Catalog Number :
201456
Product Name :
pGEX-6P-1_WT RAD23A
article :
doi10.1016/j.jmb.2023.168353
id28234221
pubmed_id37935254
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-6P-1
backbone_mutation
backbone_origin
backbone_size4984
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
GST tag is cleavable with rhinovirus 3C and PreScission proteases
growth strain :
Expresses human RAD23A with an N-terminal GST tag in E. coli
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promotertac promoter
sequencing_primer_3GCTTACAGACAAGCTGTGACC
sequencing_primer_5GGGCTGGCAAGCCACGTTTGGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHHR23A, HR23A
geneRAD23A
id5886
genbank_ids
mutationCoding sequence has been optimised for expression in E. coli
nameUV excision repair protein RAD23 homolog A
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
4679
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA