PB-TAC-ERP2-(N-HA)KRAS_G12D | Addgene 201254 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PB-TAC-ERP2-(N-HA)KRAS_G12D

catalog :

201254

citations: 1

Reference
Breunig M, Merkle J, Wagner M, Melzer M, Barth T, Engleitner T, et al. Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells. Cell Stem Cell. 2021;28:1105-1124.e19 pubmed publisher

product information

Catalog Number :

201254

Product Name :

PB-TAC-ERP2-(N-HA)KRAS_G12D

article :

doi	10.1016/j.stem.2021.03.005
id	28220575
pubmed_id	33915078

bacterial resistance :

Ampicillin

cloning :

backbone

PB-TAC-ERP2

backbone_mutation

None

backbone_origin

Knut Woltjen, Addgene plasmid #80478

backbone_size

10723

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

piggyBac transposon

growth strain :

Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

origin :

pi :

C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2geneKRASid3845genbank_ids

NM_001369787.1

NM_004985.5

mutationchanged Gly (G) 12 to Asp (D)nameKRASshRNA_sequencesize624species

9606

Homo sapiens

tags

location	N terminal on insert
tag	HA-Tag

Cloningcloning_site_3
cloning_site
_5promotertd>TREsequencing_
primer_3agaaagctgggtgtgacacaca
ttccacagggtsequencing
_primer_5aaaaagcaggcttcactagtg
tctttcataagcttatgtatccatatgatgtgcccgact
site_3_destroyed
site_5_destroyed<
/td>>entrez_gene>

aliases

'C-K-RA
S,

alt_names

Kirsten rat sarcoma viral oncogene

cloning

clone_method

Gateway

plasmid copy :

pBABE-KRAS G12D, Addgene 58902, Channing Der lab

resistance markers :

6581

tags :

High Copy

terms :

Puromycin