This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF
catalog :
201217
citations: 1
Reference
Perry Hauser N, VanDyck M, Lee K, Shi L, Javitch J. Disentangling autoproteolytic cleavage from tethered agonist-dependent activation of the adhesion receptor ADGRL3. J Biol Chem. 2022;298:102594 pubmed publisher
product information
Catalog Number :
201217
Product Name :
pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF
article :
doi10.1016/j.jbc.2022.102594
id28237816
pubmed_id36244455
bacterial resistance :
Ampicillin
cloning :
backbonepCDNA3.1
backbone_mutation
backbone_origin
backbone_size5248
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF
origin :
30
pi :
alt_names
Lphn3
Latrophilin-3
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliases5430402I23Rik, CIRL-3, D130075K09Rik, Gm1379, LEC3, Lphn3, mKIAA0768
geneAdgrl3
id319387
genbank_ids
mutation
nameADGRL3
shRNA_sequence
size2523
species
10090
Mus musculus
tags
locationN terminal on insert
tagSNAP
locationN terminal on insert
tagFlag
resistance markers :
228
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA