This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJS288
catalog :
200714
citations: 1
Reference
Fuhrmeister R, Streubel J. Functional Analysis of Plant Monosaccharide Transporters Using a Simple Growth Complementation Assay in Yeast. Bio Protoc. 2023;13:e4733 pubmed publisher
product information
Catalog Number :
200714
Product Name :
pJS288
article :
doi10.21769/BioProtoc.4733
id28234207
pubmed_id37575400
bacterial resistance :
Ampicillin
cloning :
backbonepAGT572
backbone_mutation
backbone_originSylvestre Marillonnet; Alain Tissier
backbone_size5060
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
Synthetic Biology
growth strain :
To express yeast Hxt1 hexose transporter in yeast
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsaI
cloning_site_5BsaI
promoter
sequencing_primer_3CTGCCTTTGCTGAGCTGGATC
sequencing_primer_5CCAATCTAAGTCTGTGCTCCTTCC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesYGL008C, KTI10
genePMA1
id852876
genbank_ids
mutationdomesticated for MoClo cloning (removal of internal BpiI or BsaI sites)
namePMA1 promoter
shRNA_sequence
size1008
species
4932
Saccharomyces cerevisiae
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsaI
cloning_site_5BsaI
promoter
sequencing_primer_3CTGCCTTTGCTGAGCTGGATC
sequencing_primer_5CCAATCTAAGTCTGTGCTCCTTCC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesYHR094C, HOR4
geneHXT1
id856494
genbank_ids
mutation
nameHxt1
shRNA_sequence
size1710
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tagmCherry
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsaI
cloning_site_5BsaI
promoter
sequencing_primer_3CTGCCTTTGCTGAGCTGGATC
sequencing_primer_5CCAATCTAAGTCTGTGCTCCTTCC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesYMR303C, ADR2
geneADH2
id855349
genbank_ids
CP020137.1
mutation
nameADH2 Ter
shRNA_sequence
size327
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
6491
tags :
High Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA