This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLV-EF1A-CD19.28H.28TM.28.3z
catalog :
200678
citations: 1
Reference
Timpanaro A, Piccand C, Dzhumashev D, Anton Joseph S, Robbi A, Moser J, et al. CD276-CAR T cells and Dual-CAR T cells targeting CD276/FGFR4 promote rhabdomyosarcoma clearance in orthotopic mouse models. J Exp Clin Cancer Res. 2023;42:293 pubmed publisher
product information
Catalog Number :
200678
Product Name :
pLV-EF1A-CD19.28H.28TM.28.3z
article :
doi10.1186/s13046-023-02838-3
id28234205
pubmed_id37924157
bacterial resistance :
Ampicillin
cloning :
backbonepLV
backbone_mutation
backbone_originVectorBuilder
backbone_size6451
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
origin :
37
pi :
alt_names
CD19(FMC63)-CAR, CD28 hinge, CD28 transmembrane, CD28 costim., CD3z stim domains, P2A-GFP marker
Contains: FMC63-scFV, CD28 Hinge domain, CD28-transmembrane domain, CD28-ITD, CD3z-ITD
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterEF1a-short
sequencing_primer_3tttcttccacgtcgcctgcttg
sequencing_primer_5tgatgtcgtgtactggctcc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesTp44
geneCD28
id940
aliasesCD3-ZETA, CD3H, CD3Q, CD3Z, CD3ZETA, IMD25, T3Z, TCRZ
geneCD247
id919
genbank_ids
mutation
nameFMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP
shRNA_sequence
size2334
species
9606
Homo sapiens
tags
locationN terminal on insert
tagMycTag
resistance markers :
6460
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA