This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSP1E3D1
catalog :
20066
citations: 42
Reference
St x119 pie x144 P, x15a wi x105 tek S, Robles M, Markiewicz Mizera J, Balakrishnan D, Inaba Inoue S, et al. CRAFTing Delivery of Membrane Proteins into Protocells using Nanodiscs. ACS Appl Mater Interfaces. 2023;15:56689-701 pubmed publisher
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Surya W, Yong C, Tyagi A, Bhushan S, Torres J. Anomalous Oligomerization Behavior of E. coli Aquaporin Z in Detergent and in Nanodiscs. Int J Mol Sci. 2023;24: pubmed publisher
Takahashi H, Yamada T, Denton J, Strange K, Karakas E. Cryo-EM structures of an LRRC8 chimera with native functional properties reveal heptameric assembly. elife. 2023;12: pubmed publisher
Lee H, Cha H, Jeong H, Lee S, Lee C, Kim M, et al. Conformational changes in the human Cx43/GJA1 gap junction channel visualized using cryo-EM. Nat Commun. 2023;14:931 pubmed publisher
Petroff J, Dietzen N, Santiago McRae E, Deng B, Washington M, Chen L, et al. Open-channel structure of a pentameric ligand-gated ion channel reveals a mechanism of leaflet-specific phospholipid modulation. Nat Commun. 2022;13:7017 pubmed publisher
Kumar A, Kindig K, Rao S, Zaki A, Basak S, Sansom M, et al. Structural basis for cannabinoid-induced potentiation of alpha1-glycine receptors in lipid nanodiscs. Nat Commun. 2022;13:4862 pubmed publisher
Keener J, Jayasekera H, Marty M. Investigating the Lipid Selectivity of Membrane Proteins in Heterogeneous Nanodiscs. Anal Chem. 2022;94:8497-8505 pubmed publisher
Katsube S, Liang R, Amin A, Hariharan P, Guan L. Molecular Basis for the Cation Selectivity of Salmonella typhimurium Melibiose Permease. J Mol Biol. 2022;434:167598 pubmed publisher
Bruguera E, Mahoney J, Weis W. Reconstitution of purified membrane protein dimers in lipid nanodiscs with defined stoichiometry and orientation using a split GFP tether. J Biol Chem. 2022;298:101628 pubmed publisher
Ellinghaus T, Marcellino T, Srinivasan V, Lill R, Kuhlbrandt W. Conformational changes in the yeast mitochondrial ABC transporter Atm1 during the transport cycle. Sci Adv. 2021;7:eabk2392 pubmed publisher
Julien J, Fernandez M, Brandmier K, Del Mundo J, Bator C, Loftus L, et al. Rapid preparation of nanodiscs for biophysical studies. Arch Biochem Biophys. 2021;712:109051 pubmed publisher
Grupi A, Shapira Z, Yudovich S, Degani Katzav N, Weiss S. Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs. Biophys Rep. 2021;1:None pubmed publisher
Ghilarov D, Inaba Inoue S, Stepien P, Qu F, Michalczyk E, Pakosz Z, et al. Molecular mechanism of SbmA, a promiscuous transporter exploited by antimicrobial peptides. Sci Adv. 2021;7:eabj5363 pubmed publisher
Dykstra H, LaRose C, Fisk C, Waldhart A, Meng X, Zhao G, et al. TXNIP interaction with GLUT1 depends on PI(4,5)P2. Biochim Biophys Acta Biomembr. 2021;1863:183757 pubmed publisher
Hariharan P, Guan L. Cooperative binding ensures the obligatory melibiose/Na+ cotransport in MelB. J Gen Physiol. 2021;153: pubmed publisher
Kumar A, Basak S, Chakrapani S. Recombinant expression and purification of pentameric ligand-gated ion channels for Cryo-EM structural studies. Methods Enzymol. 2021;652:81-103 pubmed publisher
Kato T, Tsukamoto T, Demura M, Kikukawa T. Real-time identification of two substrate-binding intermediates for the light-driven sodium pump rhodopsin. J Biol Chem. 2021;296:100792 pubmed publisher
Uchański T, Masiulis S, Fischer B, Kalichuk V, López Sánchez U, Zarkadas E, et al. Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM. Nat Methods. 2021;18:60-68 pubmed publisher
Kumar A, Basak S, Rao S, Gicheru Y, Mayer M, Sansom M, et al. Mechanisms of activation and desensitization of full-length glycine receptor in lipid nanodiscs. Nat Commun. 2020;11:3752 pubmed publisher
Reddy B, Bavi N, Lu A, Park Y, Perozo E. Molecular basis of force-from-lipids gating in the mechanosensitive channel MscS. elife. 2019;8: pubmed publisher
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Ogren J, Tong A, Gordon S, Chenu A, Lu Y, Blankenship R, et al. Impact of the lipid bilayer on energy transfer kinetics in the photosynthetic protein LH2. Chem Sci. 2018;9:3095-3104 pubmed publisher
Srivastava A, Luo M, Zhou W, Symersky J, Bai D, Chambers M, et al. High-resolution cryo-EM analysis of the yeast ATP synthase in a lipid membrane. Science. 2018;360: pubmed publisher
Gutmann T, Kim K, Grzybek M, Walz T, Coskun U. Visualization of ligand-induced transmembrane signaling in the full-length human insulin receptor. J Cell Biol. 2018;217:1643-1649 pubmed publisher
B Kumar R, Zhu L, Hebert H, Jegerschöld C. Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy. J Vis Exp. 2017;: pubmed publisher
Stam N, Wilkens S. Structure of the Lipid Nanodisc-reconstituted Vacuolar ATPase Proton Channel: DEFINITION OF THE INTERACTION OF ROTOR AND STATOR AND IMPLICATIONS FOR ENZYME REGULATION BY REVERSIBLE DISSOCIATION. J Biol Chem. 2017;292:1749-1761 pubmed publisher
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Ding Y, Fujimoto L, Yao Y, Marassi F. Solid-state NMR of the Yersinia pestis outer membrane protein Ail in lipid bilayer nanodiscs sedimented by ultracentrifugation. J Biomol NMR. 2015;61:275-86 pubmed publisher
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product information
Catalog Number :
20066
Product Name :
pMSP1E3D1
article :
doi10.1074/jbc.M609589200
id2391
pubmed_id17213193
bacterial resistance :
Kanamycin
cloning :
backbonepET 28a
backbone_mutation
backbone_originNovagen
backbone_size5300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation
growth temp :
BL-21(DE3); high oxygenation level
origin :
37
pi :
alt_names
membrane scaffold protein
cloning
clone_methodRestriction Enzyme
cloning_site_3Hind III
cloning_site_5Nco I
promoter
sequencing_primer_3T7 term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation extended MSP1D1; contains repeats of helices 4, 5 and 6;N-terminal 7-his tag followed by spacer sequence and TEV protease cleavage site
nameMSP1E3D1
shRNA_sequence
size840
species
32644
Other
synthetic gene
tags
locationN terminal on insert
tag7-His
resistance markers :
485
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA