This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSP1E3
catalog :
20064
citations: 8
Reference
Gao X, Schmidpeter P, Berka V, Durham R, Fan C, Jayaraman V, et al. Gating intermediates reveal inhibitory role of the voltage sensor in a cyclic nucleotide-modulated ion channel. Nat Commun. 2022;13:6919 pubmed publisher
Falzone M, Feng Z, Alvarenga O, Pan Y, Lee B, Cheng X, et al. TMEM16 scramblases thin the membrane to enable lipid scrambling. Nat Commun. 2022;13:2604 pubmed publisher
Schmidpeter P, Rheinberger J, Nimigean C. Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel. Nat Commun. 2020;11:6401 pubmed publisher
Wang X, Boudker O. Large domain movements through the lipid bilayer mediate substrate release and inhibition of glutamate transporters. elife. 2020;9: pubmed publisher
Fan C, Sukomon N, Flood E, Rheinberger J, Allen T, Nimigean C. Ball-and-chain inactivation in a calcium-gated potassium channel. Nature. 2020;580:288-293 pubmed publisher
Khelashvili G, Falzone M, Cheng X, Lee B, Accardi A, Weinstein H. Dynamic modulation of the lipid translocation groove generates a conductive ion channel in Ca2+-bound nhTMEM16. Nat Commun. 2019;10:4972 pubmed publisher
Rheinberger J, Gao X, Schmidpeter P, Nimigean C. Ligand discrimination and gating in cyclic nucleotide-gated ion channels from apo and partial agonist-bound cryo-EM structures. elife. 2018;7: pubmed publisher
Denisov I, Grinkova Y, Lazarides A, Sligar S. Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size. J Am Chem Soc. 2004;126:3477-87 pubmed
product information
Catalog Number :
20064
Product Name :
pMSP1E3
article :
doi10.1021/ja0393574
id2390
pubmed_id15025475
bacterial resistance :
Kanamycin
cloning :
backbonepET 28a
backbone_mutation
backbone_originNovagen
backbone_size5300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth temp :
BL-21(DE3); high oxygenation level
origin :
37
pi :
alt_names
membrane scaffold protein
APOA1
cloning
clone_methodRestriction Enzyme
cloning_site_3Hind III
cloning_site_5Nco I
promoter
sequencing_primer_3T7 term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHPALP2, apo(a)
geneAPOA1
id335
genbank_ids
mutation extended MSP1; contains repeats of helices 4, 5 and 6
nameMSP1E3
shRNA_sequence
size870
species
synthetic gene
tags
locationN terminal on insert
tag6-His
resistance markers :
485
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA