pMSP1 | Addgene 20060 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMSP1

catalog :

20060

citations: 4

Reference
Liu X, Xu X, Hilger D, Aschauer P, Tiemann J, Du Y, et al. Structural Insights into the Process of GPCR-G Protein Complex Formation. Cell. 2019;177:1243-1251.e12 pubmed publisher
Du Y, Duc N, Rasmussen S, Hilger D, Kubiak X, Wang L, et al. Assembly of a GPCR-G Protein Complex. Cell. 2019;177:1232-1242.e11 pubmed publisher
Komolov K, Du Y, Duc N, Betz R, Rodrigues J, Leib R, et al. Structural and Functional Analysis of a β2-Adrenergic Receptor Complex with GRK5. Cell. 2017;169:407-421.e16 pubmed publisher
Denisov I, Grinkova Y, Lazarides A, Sligar S. Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size. J Am Chem Soc. 2004;126:3477-87 pubmed

product information

Catalog Number :

20060

Product Name :

pMSP1

article :

doi	10.1021/ja0393574
id	2390
pubmed_id	15025475

bacterial resistance :

Kanamycin

cloning :

backbone

pET 28a

backbone_mutation

backbone_origin

Novagen

backbone_size

5300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

First described in the following paper: Bayburt, T. H., Grinkova, Y. V., & Sligar, S. G., 2002, Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins. Nanoletters, 2: 853-856.

growth temp :

BL-21(DE3); high oxygenation level

origin :

pi :

alt_names

membrane scaffold protein;

apolipoprotein A-1

APOA1

cloning

clone_method	Restriction Enzyme
cloning_site_3	Hind III
cloning_site_5	Nco I
promoter
sequencing_primer_3	T7 term
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HPALP2, apo(a)
gene	APOA1
id	335

genbank_ids

mutation

synthetic gene of deletion mutant (1-43) of human Apolipoprotein A-1; contains N-terminal 6-His tag and Factor X site; the codons optimized for E. coli expression

name

MSP1

shRNA_sequence

size

640

species

synthetic gene