This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
H6GB1tevP53(1-303)IntN
catalog :
200312
citations: 1
Reference
Krois A, Park S, Martinez Yamout M, Dyson H, Wright P. Mapping Interactions of the Intrinsically Disordered C-Terminal Regions of Tetrameric p53 by Segmental Isotope Labeling and NMR. Biochemistry. 2022;61:2709-2719 pubmed publisher
product information
Catalog Number :
200312
Product Name :
H6GB1tevP53(1-303)IntN
article :
doi10.1021/acs.biochem.2c00528
id28234147
pubmed_id36380579
bacterial resistance :
Ampicillin
cloning :
backbonepET21a
backbone_mutation
backbone_origin
backbone_size5443
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The intein sequence was taken from Addgene #20178
growth strain :
Bacterial expression of P53 NTAD-DBD for segmental labeling
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NcoI
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutationM133L, V203A, N239Y, N268D
namep53
shRNA_sequence
size1922
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHis6GB1tev
locationC terminal on insert
tagdesigned intein N
resistance markers :
3524
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA