This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-FLAG-Rheb-N153T
catalog :
19997
citations: 9
Reference
Gong Y, Shao H, Li Y, Brafford P, Stine Z, Sun J, et al. Na+/H+-exchanger 1 enhances antitumor activity of engineered NK-92 natural killer cells. Cancer Res Commun. 2022;2:842-856 pubmed publisher
Ammanathan V, Mishra P, Chavalmane A, Muthusamy S, Jadhav V, Siddamadappa C, et al. Restriction of intracellular Salmonella replication by restoring TFEB-mediated xenophagy. Autophagy. 2019;:1-14 pubmed publisher
Demian W, Persaud A, Jiang C, Coyaud E, Liu S, Kapus A, et al. The Ion Transporter NKCC1 Links Cell Volume to Cell Mass Regulation by Suppressing mTORC1. Cell Rep. 2019;27:1886-1896.e6 pubmed publisher
Li R, Xu J, Fu C, Zhang J, Zheng Y, Jia H, et al. Regulation of mTORC1 by lysosomal calcium and calmodulin. elife. 2016;5: pubmed publisher
Correia Melo C, Marques F, Anderson R, Hewitt G, Hewitt R, Cole J, et al. Mitochondria are required for pro-ageing features of the senescent phenotype. EMBO J. 2016;35:724-42 pubmed publisher
Carroll B, Maetzel D, Maddocks O, Otten G, Ratcliff M, Smith G, et al. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. elife. 2016;5: pubmed publisher
Durán R, MacKenzie E, Boulahbel H, Frezza C, Heiserich L, Tardito S, et al. HIF-independent role of prolyl hydroxylases in the cellular response to amino acids. Oncogene. 2013;32:4549-56 pubmed publisher
Durán R, Oppliger W, Robitaille A, Heiserich L, Skendaj R, Gottlieb E, et al. Glutaminolysis activates Rag-mTORC1 signaling. Mol Cell. 2012;47:349-58 pubmed publisher
Urano J, Sato T, Matsuo T, Otsubo Y, Yamamoto M, Tamanoi F. Point mutations in TOR confer Rheb-independent growth in fission yeast and nutrient-independent mammalian TOR signaling in mammalian cells. Proc Natl Acad Sci U S A. 2007;104:3514-9 pubmed
product information
Catalog Number :
19997
Product Name :
pcDNA3-FLAG-Rheb-N153T
article :
doi10.1073/pnas.0608510104
id2082
pubmed_id17360675
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
Rheb-N153T
RHEB2; MGC111559; RHEB
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRHEB2
geneRHEB
id6009
genbank_ids
mutationChanged Asparagine 153 to Threonine
nameRas homolog enriched in brain 1 - N153T
shRNA_sequence
size600
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
resistance markers :
517
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA