This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-FLAG-Rheb
catalog :
19996
citations: 9
Reference
Ma S, Dong Z, Huang Y, Liu J, Zhang J. Translation initiation factor eIF3a regulates glucose metabolism and cell proliferation via promoting small GTPase Rheb synthesis and AMPK activation. J Biol Chem. 2022;298:102044 pubmed publisher
da Silva Rosa S, Martens M, Field J, Nguyen L, Kereliuk S, Hai Y, et al. BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation. Autophagy. 2020;:1-16 pubmed publisher
Viscarra J, Wang Y, Nguyen H, Choi Y, Sul H. Histone demethylase JMJD1C is phosphorylated by mTOR to activate de novo lipogenesis. Nat Commun. 2020;11:796 pubmed publisher
Makhoul C, Gosavi P, Duffield R, Delbridge B, Williamson N, Gleeson P. Intersectin-1 interacts with the golgin GCC88 to couple the actin network and Golgi architecture. Mol Biol Cell. 2019;30:370-386 pubmed publisher
Nguyen T, Nokin M, Egorov M, Tomé M, Bodineau C, Di Primo C, et al. mTOR Inhibition via Displacement of Phosphatidic Acid Induces Enhanced Cytotoxicity Specifically in Cancer Cells. Cancer Res. 2018;78:5384-5397 pubmed publisher
Gorbunova E, Simons M, Gavrilovskaya I, Mackow E. The Andes Virus Nucleocapsid Protein Directs Basal Endothelial Cell Permeability by Activating RhoA. MBio. 2016;7: pubmed publisher
Ghosh A, Marshall C, Coric T, Shim E, Kirkman R, Ballestas M, et al. Point mutations of the mTOR-RHEB pathway in renal cell carcinoma. Oncotarget. 2015;6:17895-910 pubmed
Xie J, Ponuwei G, Moore C, Willars G, Tee A, Herbert T. cAMP inhibits mammalian target of rapamycin complex-1 and -2 (mTORC1 and 2) by promoting complex dissociation and inhibiting mTOR kinase activity. Cell Signal. 2011;23:1927-35 pubmed publisher
Urano J, Sato T, Matsuo T, Otsubo Y, Yamamoto M, Tamanoi F. Point mutations in TOR confer Rheb-independent growth in fission yeast and nutrient-independent mammalian TOR signaling in mammalian cells. Proc Natl Acad Sci U S A. 2007;104:3514-9 pubmed
product information
Catalog Number :
19996
Product Name :
pcDNA3-FLAG-Rheb
article :
doi10.1073/pnas.0608510104
id2082
pubmed_id17360675
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
Rheb
RHEB2; MGC111559; RHEB
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRHEB2
geneRHEB
id6009
genbank_ids
mutation
nameRas homolog enriched in brain 1
shRNA_sequence
size600
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
resistance markers :
517
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA